期刊
PROTEOMICS
卷 6, 期 15, 页码 4309-4320出版社
WILEY
DOI: 10.1002/pmic.200500907
关键词
2-DE reference map; Chlamydomonas reinhardti; comparative proteomics; high light stress; photoprotection
High light (HL) stress adversely affects growth, productivity and viability of photosynthetic organisms. The green alga Chlamydomonas reinhardtii is a model system to study photosynthesis and light stress. Comparative proteomics of wild-type and two very high light (VHL)-resistant mutants, VHLR-S4 and VHLR-S9, revealed complex alterations in response to excess light. A two-dimensional reference map of the soluble subproteome was constructed representing about 1500 proteins. A total of 83 proteins from various metabolic pathways were identified by peptide mass fingerprinting. Quantitative comparisons of 444 proteins showed 105 significantly changed proteins between wild type and mutants under different light conditions. Commonly, more proteins were decreased than increased, but different proteins were affected in each genotype. Proteins uniquely altered in either VHLR mutant may be involved in VHL resistance. Such candidate proteins similarly altered without light stress, thus possibly contributing to pre-adaptation of mutants to VHL, included decreased levels of a DEAD box RNA helicase (VHLR-S4) and NAB1 and RB38 proteins (VHLR-S9), and increased levels of an oxygen evolving enhancer 1 (OEE1) isoform. and an unknown protein (VHLR-S4). Changes from increased levels in HL to decreased levels in excess light, included OEE1 (VHLR-Sg) or the reverse change for NAB1, RB38, beta-carbonic anhydrase and an ABC transporter-like protein (VHLR-S4).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据