期刊
PLANT AND CELL PHYSIOLOGY
卷 47, 期 8, 页码 1175-1181出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcj086
关键词
actin filament; dynamin; microtubule; microtubule-associated protein (MAP); NtDRP3; tobacco BY-2 cell
We purified an 84 kDa polypeptide from the MAP (microtubule-associated protein) fraction of tobacco BY-2 cultured cells. LC/MS/MS (liquid chromatography-tandem mass spectrometry) analysis revealed that this polypeptide is a tobacco homolog of AtDRP3 (Arabidopsis thaliana dynamin-related protein 3). Electron microscopy revealed that NtDRP3 (Nicotiana tabacum dynamin-related protein 3) assembles to form a filamentous structure. When GDP was added to the NtDRP3 fraction, the filaments disappeared and many particles appeared. Biochemical analysis revealed that NtDRP3 could bind to and bundle both microtubules and actin filaments in vitro.
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