Bioeffects of different functionalized silica nanoparticles on HaCaT cell line

The bioeffects of silica nanoparticles (SiNP), phosphorylate-terminated nanoparticles (PO4-NP) and amino-terminated nanoparticles (NH2NP) on HaCaT cell line have been studied in this paper. The effects of the three kinds of functionalized silica nanoparticles on adherence, proliferation and cycle of HaCaT cells have been investigated. And the cellular uptake of the three kinds of functionalized silica nanoparticles by HaCaT cells has also been examined. Results indicated that the bioeffects of the three kinds of functionalized nanoparticles on HaCaT cells were concentration-dependent. And the three kinds of functionalized nanoparticles all exhibited well biocompatibility if the concentration was below 0.2 mu g/gL. While the cytotoxicities of the three kinds of functionalized nanoparticles on HaCaT cells would increase with the increasing of nanoparticles concentration, and the following order was observed: NH2NP > SiNP > PO4NP. In addition, the quantity and rapidity of cellular uptake of nanoparticles by HaCaT cells were diverse due to the different functional groups. Under the same conditions, NH2NP was most and fast internalized by HaCaT cells, followed by SiNP, and PO4NP was the least and slowest. These results provided theoretical foundation for the safe application and further modification of silica nanoparticles, which could broaden the application of silica nanoparticles in biomedicine.