Comparative evaluation of three high-performance liquid chromatography-based Kow estimation methods for highly hydrophobic organic compounds:: Polybrominated diphenyl ethers and hexabromocyclododecane

Three methods for estimating the octanol-water partition coefficient (K-ow) based on its relationship with capacity factors on reversed-phase (RP) high-performance liquid chromatography (HPLC) columns were compared in terms of their applicability to highly hydrophobic compounds (HHCs). Methods based on simple isocratic elutions were found to be unsuitable, because the very high organic modifier fractions that are required to elute HHCs from RP columns challenge the basic assumption of the similarity between the octanol-water and RP-eluent systems. Compound planarity was found to exert a considerable influence on the retention of HHCs in RP columns, leading to different linear calibration curves for chlorobenzenes and chlorobiphenyls. Only an empirical exponential regression succeeded in describing the behavior of both groups of compounds during gradient elutions. In a method based on isocratic retention times at multiple temperatures, satisfactory calibration was achieved with a multivariate linear regression that included a numerical indicator of compound planarity. Considering experimental simplicity, speed, precision, and accuracy, with the latter judged by comparison with K-ow values for polybrominated diphenyl ethers and polychlorinated naphthalenes as reported in the literature, a gradient elution combined with an exponential calibration curve is recommended for estimating the K-ow of HHCs. To our knowledge, the first isomer-specific K., values for hexabromocyclododecane are reported. Bearing in mind that the influence of structural characteristics on retention is likely to increase with hydrophobicity, it is not justified to judge a HPLC-based K-ow estimation method as being suitable for HHCs because it is shown to work well for less hydrophobic substances. Whereas univariate linear regressions often may prove to be sufficient when dealing with substances having a log K-ow of less than five, methods for HHCs need to account for the influence of structure on retention.