期刊
JOURNAL OF IMMUNOLOGY
卷 177, 期 3, 页码 1581-1589出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.177.3.1581
关键词
-
类别
资金
- NCI NIH HHS [CA 34233, CA 33399] Funding Source: Medline
- NHLBI NIH HHS [N01-HV-281831] Funding Source: Medline
Differences in BCR signaling may govern outcomes as diverse as proliferation and cell death. We profiled BCR signaling kinetics in subsets of primary human B cells using flow cytometry. In the predominant population expressing IgM, BCR cross-linking led to a quick burst of Syk, ERK1/2, and p38 signaling. In contrast, IgG B cells sustained higher per-cell ERKI/2 phosphorylation over time. This dichotomy suggested a mechanism for dampening signals transmitted by IgM. Regulatory phosphatase activity in IgM B cells was BCR-mediated and initiated more slowly than kinase activity. This BCR-mediated phosphatase activity was sensitive to inhibition by H2O2, and required to attenuate IgM BCR signaling. These results provide the first kinetic maps of BCR signaling in primary human B cell subsets and enable new studies of signaling in B cell disorders, such as autoimmunity and cancer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据