期刊
BIOTECHNOLOGY LETTERS
卷 28, 期 15, 页码 1181-1185出版社
SPRINGER
DOI: 10.1007/s10529-006-9069-8
关键词
high-throughput screening; lipase; enantioselective; growth-based screening; pro-antibiotic substrate
A new growth-based screening method for the identification of enantioselective hydrolases, such as lipases and esterases, using pro-antibiotic substrates was devised. An enantioselective hydrolase could be identified by measuring growth rates of cells in liquid media containing (R)- or (S)-2-phenylbutyric chloramphenicol esters. This method can be applied to the screening of novel enantioselective microbes and to the high-throughput screening for the directed evolution of enantioselective hydrolytic enzymes.
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