期刊
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
卷 102, 期 2, 页码 135-137出版社
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1263/jbb.102.135
关键词
flavonoids; glucosylation; Oryza sativa
A glucosyltransferase cDNA, RF5, was cloned from Oryza sativa using an RT-PCR strategy and expressed in Escherichia coli. Several flavonoids were tested for their ability to serve as substrates for RF5. RF5 effectively glucosylated kaempferol and quercetin to produce their 3-O-glucosides. Thus, RF5 could be defined as a flavonol 3-O-glucosyltransferase. E. coli cells expressing RF5 effectively converted 100 mu M of kaempferol and quercetin into their corresponding glucosides.
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