期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 385, 期 8, 页码 1504-1512出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-006-0550-y
关键词
selenium; speciation; anion exchange chromatography; experimental design
We describe a new method for separating the organic and inorganic selenocompounds methaneseleninic acid, selenite, selenate, methylselenocysteine, selenocystine as well as both selenomethionine and its oxidized form. The separation is performed on a Hamilton PRP-X100 column. According to the literature, the oxidized form of selenomethionine-which is easily formed-is eluted close to the dead volume when this column is used. The choice of parahydroxybenzoic acid as mobile phase enabled us to elute all of these species after this oxidized form, resulting in better identification and quantification. The factors determining separation (eluent concentration, pH, gradient) were optimized via an experimental design. Application of the method to yeast and commercial tablets showed that the principal Se compound present was selenomethionine, which was also present in its oxidized form.
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