期刊
GENE THERAPY
卷 13, 期 15, 页码 1188-1190出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.gt.3302789
关键词
nonviral gene transfer; site-specific integration; chromosomal instability
phi c31 integrase is investigated as a novel tool for nonviral gene therapy as the enzyme can direct site-specific integration into a host chromosome. In order to investigate effects of phi c31 integrase expression in normal human cells, we have generated stably transfected primary human fibroblasts expressing the enzyme. All control cells were cytogenetically normal, but in cells expressing fc31 integrase, numerous chromosomal abnormalities including various translocations were found, suggesting that the enzyme itself acts as a mutagen.
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