期刊
CRYOBIOLOGY
卷 53, 期 1, 页码 143-147出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cryobiol.2006.05.002
关键词
Amphora coffeaeformis; cryoinjury; cryopreservation; diatoms; dimethyl sulfoxide; Navicula delicatula; post-preservation recovery
Two permate microphytobenthic diatoms, Amphora coffeaeformis (Agardh) Kutzing and Navicula transitans var. derasa f. delicatula Heimdal, were cryopreserved and monitored on thawing to track the mechanical injuries and their post-preservation recovery. Cells were subjected to (1) direct freezing in liquid nitrogen and (2) two-step cooling with and without the cryoprotectant, dimethyl sulfoxide (Me,SO). Mechanical injury due to exposure to low temperature differed between the two species. While A. coffeaeformis cells were intact and could survive even direct freezing without a cryoprotectant, N. delicatula cell chloroplasts were damaged. However, the two-step cooling along with a cryoprotectant minimized the mechanical injury to cells of both species thereby enhancing the post-thaw viability. (c) 2006 Elsevier Inc. All rights reserved.
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