期刊
JOURNAL OF COLLOID AND INTERFACE SCIENCE
卷 300, 期 1, 页码 117-122出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2006.03.063
关键词
poly(phenylene ethynylene); nanoparticle; fluorescence quenching; Stern-Volmer analysis; nucleic acid detection
Fabrication, characterization, and application of poly(phenylene ethynylene) (PPE)/silica composite particles are described. PPE is a class of conjugated polymers, which has been used for various sensory materials. However, its hydrophobic nature makes its application difficult in the aqueous phase. especially for biological substance detection. In this report, we utilized non-aqueous soluble PPE, 15 nm of colloidal silica particles, and aminosilane to fabricate a biosensory platform. The resulting composite showed high aqueous compatibility, large surface area, high quantum efficiency, and versatile chemical modification including oligonucleotide coupling. By monitoring the fluorescence quenching of PPE, we could detect a quencher-labeled target oligonucleotide specifically. Stern-Volmer (SV) analysis showed different accessibility of fluorophores (PPE) to a quencher labeled target oligonucleotide. The accessibility of fluorophores and SV constant are determined to be 0.54 and 4.2 x 10(7) M-1, respectively, from a modified SV plot. This method will broaden the capability of conjugated polymers for the sensitive detection of biological substances. (c) 2006 Elsevier Inc. All rights reserved.
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