期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 36, 期 8, 页码 634-641出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2006.05.005
关键词
fatty acid; sex pheromone; double bond; stereochemistry; moth; lepidoptera
In the biosynthetic pathway of Spodoptera littoralis sex pheromone, (E, E)- 10,12-tetradecadienoic acid is produced from (Z)-11-tetradecenoic acid by desaturation and concomitant migration of the precursor double bond. With the aim of identifying the enzyme involved in this biotransformation, yeast Delta elo1/Delta ole mutants, which are both elongase 1 and Delta(9) desaturase-deficient, were transformed with the S. littoralis Delta(11) desaturase gene using a Cu+2 inducible expression vector. The transformants produced a recombinant polyhistidine-tagged All desaturase that could be detected by immunoblotting from cell lysates. Lipid analysis revealed that besides producing large quantities of C11-monounsaturated fatty acids, mainly (Z)-11-hexadecenoic acid, (E,E)-10,12-tetradecadienoic acid and minor amounts of (EZ)-10,12-hexadecadienoic acid were also produced, as well as very low quantities of another tetradecadienoate, which was tentatively identified as the (E,Z)-10, 12-tetradecadienoic isomer. None of these dienes was detected with the Delta(11) desaturase gene of Trichoplusia ni, which does not produce conjugated dienes as pheromone components. We conclude that the Delta(11) desaturase of S. littoralis is a bifunctional enzyme with both Delta(11) and Delta(10,12) desaturation activities. The relationship between the substrate structure and the stereochemical outcome of the reaction is discussed. (c) 2006 Elsevier Ltd. All rights reserved.
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