期刊
JOURNAL OF VIROLOGY
卷 80, 期 15, 页码 7491-7499出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00435-06
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资金
- NCI NIH HHS [P01 CA013106, CA 13106] Funding Source: Medline
The El protein from papillomaviruses is a multifunctional protein with complex functions required for the initiation of viral DNA replication. We have performed a surface mutagenesis of the well-characterized E1 DNA binding domain (DBD). We demonstrate that substitutions of multiple residues on the surface of the E1 DBD are defective for DNA replication without affecting the DNA binding activity of the protein. The defects of individual substitutions include failure to form the double trimer that melts the ori and failure to form the double hexamer that unwinds the ori. These results demonstrate that the DBD plays an essential role in multiple DNA replication-related processes apart from DNA binding.
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