期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 103, 期 31, 页码 11754-11759出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0604744103
关键词
essential lipoprotein; Gram-negative bacteria; outer-membrane biogenesis
资金
- NIGMS NIH HHS [R01 GM034821, GM66174, R37 GM034821, GM34821, R01 GM066174] Funding Source: Medline
The outer membrane of most Gram-negative bacteria is made up of LIPS, and in nearly all bacteria that contain LIPS it is essential for the life of the organism. The lipid portion of this molecule, lipid A, also known as endotoxin, is a potent activator of the innate immune response. More than 50 genes are required to synthesize LIPS and assemble it at the cell surface. Enormous progress has been made in elucidating the structure and biosynthesis of LPS, but until recently the cellular components required for its transport from its site of synthesis in the inner membrane to its final cellular location at the cell surface remained elusive. Here we describe the identification of a protein complex that functions to assemble LPS at the surface of the cell. This complex contains two proteins: Imp, already identified as an essential outer-membrane protein implicated in LPS assembly; and another protein, RlpB, heretofore identified only as a rare lipoprotein. We show that RlpB is also essential for cell viability and that the Imp/RlpB complex is responsible for LIPS reaching the outer surface of the outer membrane.
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