期刊
BIOMEDICAL CHROMATOGRAPHY
卷 20, 期 8, 页码 743-747出版社
WILEY
DOI: 10.1002/bmc.590
关键词
SPE; HPLC; geniposide; Yin-Zhi-Ku decoction
A new HPLC method for the determination of geniposide in rat serum with solid-phase extraction (SPE) for preconcentration is described. Geniposide and an internal standard (paeoniflorin) were extracted from serum by SPE using C-18 cartridges. Analysis of the extract was then performed on a reversed-phase C-18 column using acetonitrile-water (16:84, v/v) as the eluting solvent system, and UV detection at 238 nm was used to measure the analyte with a limit of quantitation about 0.1 mu g/mL. The calibration curve for geniposide was linear (r = 0.9993) in the concentration range 0.1-16.0 mu g/mL. The intra- and inter-day precision of the geniposide were determined and their RSD did not exceed 10%. The validated method has been successfully applied for pharmacokinetic studies of geniposide from rat serum after oral administration of Yin-Zhi-Ku decoction. Copyright (c) 2005 John Wiley & Sons, Ltd.
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