期刊
JOURNAL OF PROTEOME RESEARCH
卷 5, 期 8, 页码 1916-1923出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr060114v
关键词
drug discovery; NMR; metabolomics; principle component analysis; Aspergillus nidulans; purine degradation pathway; pyrimidine biosynthetic pathway
资金
- NCRR NIH HHS [RR015468-01, P20 RR16469] Funding Source: Medline
We describe a general protocol for using comparative NMR metabolomics data to infer in vivo efficacy, specificity and toxicity of chemical leads within a drug discovery program. The methodology is demonstrated using Aspergillus nidulans to monitor the activity of urate oxidase and orotidine-5'-phosphate decarboxylase and the impact of 8-azaxanthine, an inhibitor of urate oxidase. 8-azaxanthine is shown to inhibit A. nidulans hyphal growth by in vivo inactivation of urate oxidase.
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