期刊
NEUROSCIENCE LETTERS
卷 404, 期 1-2, 页码 87-92出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2006.05.061
关键词
membrane depolarization; proliferation; neuroblastoma cells; retinoblastoma protein; cyclin-dependent kinases; cyclins
Membrane depolarization causes transmembrane ionic influxes that induce various gene expressions, and is involved in the processes of neuronal differentiation and apoptosis. However, the effect of membrane depolarization on neuronal proliferation has not been established. In this study, we aimed to investigate the effect of membrane depolarization on the proliferation of SH-SY5Y human neuroblastoma cells. Membrane depolarization induced by 50 mM KCI for 5 min significantly increased SH-SY5Y cell numbers and thymidine incorporation at 24 h after depolarization, and increased the phosphorylation and expression of retinoblastoma protein (RB), the activity of Cdk2 (without changing the activities of Cdk4 and Cdk6), and the expressions of cyclin A and cyclin E. Single and repeated depolarization (once a day for 6 days) had similar effects on RB, Cdks, and cyclins levels and activities. In summary, our results suggest that membrane depolarization may stimulate cellu tar proliferation by augmenting the expression of cyclin E leading to increases in Cdk2 activity and RB phosphorylation. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
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