期刊
JOURNAL OF NEUROSCIENCE
卷 26, 期 33, 页码 8578-8587出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.2185-06.2006
关键词
sensory neuron; skin; pain; thermal sensitivity; gene expression; DRG
资金
- NIDDK NIH HHS [DK063922] Funding Source: Medline
- NINDS NIH HHS [NS31826, NS23725, NS33730, R01 NS033730-13, R01 NS033730] Funding Source: Medline
Artemin, a neuronal survival factor in the glial cell line-derived neurotrophic factor family, binds the glycosylphosphatidylinositol-anchored protein GFR alpha 3 and the receptor tyrosine kinase Ret. Expression of the GFR alpha 3 receptor is primarily restricted to the peripheral nervous system and is found in a subpopulation of nociceptive sensory neurons of the dorsal root ganglia (DRGs) that coexpress the Ret and TrkA receptor tyrosine kinases and the thermosensitive channel TRPV1. To determine how artemin affects sensory neuron properties, transgenic mice that overexpress artemin in skin keratinocytes (ART-OE mice) were analyzed. Expression of artemin caused a 20.5% increase in DRG neuron number and increased the level of mRNA encoding GFR alpha 3, TrkA, TRPV1, and the putative noxious colddetecting channel TRPA1. Nearly all GFR alpha 3-positive neurons expressed TRPV1 immunoreactivity, and most of these neurons were also positive for TRPA1. Interestingly, acid-sensing ion channel (ASIC) 1, 2a, 2b, and 3 mRNAs were decreased in the DRG, and this reduction was strongest in females. Analysis of sensory neuron physiological properties using an ex vivo preparation showed that cutaneous C-fiber nociceptors of ART-OE mice had reduced heat thresholds and increased firing rates in response to a heat ramp. No change in mechanical threshold was detected. Behavioral testing of ART-OE mice showed that they had increased sensitivity to both heat and noxious cold. These results indicate that the level of artemin in the skin modulates gene expression and response properties of afferents that project to the skin and that these changes lead to behavioral sensitivity to both hot and cold stimuli.
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