期刊
IMMUNOLOGY LETTERS
卷 106, 期 2, 页码 135-143出版社
ELSEVIER
DOI: 10.1016/j.imlet.2006.05.003
关键词
dendritic cells; costimulation; cell cycle
类别
Costimulation by members of the B7 family of molecules is critical for the activation of naive CD4(+) T cells. While prolonged TCR signaling is necessary for T cell activation, the duration of costimulatory signals required has not been established. In this study, murine bone marrow-derived dendritic cells (DC) and naive CD4(+) T cells were used to determine the temporal costimulatory requirements for naive T cell activation. By blocking CD80/CD86 costimulation at various time points during DC-T cell interaction and using the CFSE technique to assess the dynamics of T cell proliferation, we found that prolonged costimulation was required for naive T cells to enter and progress through the cell cycle over a wide range of peptide concentrations. Prolonged costimulation was also important for IL-2 production and CD25/CD69 expression by naive T cells. Video microscopy demonstrated that DC and naive T cells formed stable conjugates that persisted for more than 6 h. Thus, persistent CD80/CD86 signaling during prolonged interactions with DC allows naive T cells to enter the cell cycle and programs the daughter cells to undergo subsequent divisions. (c) 2006 Elsevier B.V. All rights reserved.
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