期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 840, 期 2, 页码 108-115出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2006.04.044
关键词
vorinostat; suberoylanilide hydroxamic acid; SAHA; histone deacetylase inhibitor; LC-MS/MS
资金
- NCI NIH HHS [N01-CO-124001, 2P30 CA 47904, U01-CA099168] Funding Source: Medline
Vorinostat (suberoylanilide hydroxamic acid, SAHA) is undergoing evaluation as an antineoplastic agent. We developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for quantitating vorinostat and its major metabolites, vorinostat glucuronide and 4-anilino-4-oxobutanoic acid, in human serum. The assay uses: deuterated internal standards; acetonitrile protein precipitation; a BDS Hypersil C18 (3 mu m, 100 mm x 3 mm) column; a gradient mobile phase of 0.5% acetic acid in acetonitrile and water; and electrospray positive-mode ionization with selected reaction monitoring (SRM) detection. The lower limit of quantitation was 3.0 ng/ml for each analyte. The assay is being employed in at least 12 clinical studies of vormostat-containing regimens. (c) 2006 Elsevier B.V. All rights reserved.
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