期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 94, 期 6, 页码 1209-1213出版社
WILEY
DOI: 10.1002/bit.20928
关键词
biocompatible ionic liquids; protein stability; activity; secondary structure; biopreservation
Proteins generally are only stable in vitro for short periods of time. This results in challenges during isolation and purification of recombinant proteins and reduces the shelf life of protein-based pharmaceuticals. Here we show that certain novel, biocompatible ionic liquids provide a stabilizing solvent for proteins, for example, cytochrome c, such that structure and activity are maintained even after 6 months of storage at room temperature. Normally, this protein would be rendered inactive after only 1 week in buffered aqueous solution. The effect of the ionic liquid solvent appears to be related to protection against hydrolysis. (c) 2006 Wiley Periodicals, Inc.
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