期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 103, 期 34, 页码 12707-12712出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0605686103
关键词
yeast Saccharomyces cerevisiae
资金
- NCRR NIH HHS [P41 RR011823, RR11823-09] Funding Source: Medline
- NIGMS NIH HHS [R01 GM035949, R01 GM063952, GM-35949, GM-63952] Funding Source: Medline
Previous investigations into the mechanisms that control RNA Polymerase (Pol) I transcription have primarily focused on the process of transcription initiation, thus little is known regarding postinitiation steps in the transcription cycle. Spt4p and Spt5p are conserved throughout eukaryotes, and they affect elongation by Pol II. We have found that these two proteins copurify with Pol I and associate with the rDNA in vivo. Disruption of the gene for Spt4p resulted in a modest decrease in growth and rRNA synthesis rates at the permissive temperature, 30 degrees C. Furthermore, biochemical and EM analyses showed clear defects in rRNA processing. These data suggest that Spt4p, Spt5p, and, potentially, other regulators of Pol I transcription elongation play important roles in coupling rRNA transcription to its processing and ribosome assembly.
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