4.7 Article

Major effects of sensory experiences on the neocortical inhibitory circuits

期刊

JOURNAL OF NEUROSCIENCE
卷 26, 期 34, 页码 8691-8701

出版社

SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.2478-06.2006

关键词

development; plasticity; barrel cortex; interneuron; GABA; somatosensory

资金

  1. NCRR NIH HHS [P20 RR16474-04, P20 RR015553, RR15640, P20 RR015640, P20 RR016474] Funding Source: Medline
  2. NINDS NIH HHS [R01 NS057415, R01 NS057415-01A1] Funding Source: Medline
  3. PHS HHS [P20 RRO15553] Funding Source: Medline

向作者/读者索取更多资源

During postnatal development, sensory experiences play critical roles in the refinement of cortical connections. However, both the process of postnatal experience-dependent maturation of neocortical inhibitory networks and its underlying mechanisms remain elusive. Here, we examined the differential properties of intracortical inhibitory networks of layer IV in sensory-spared and sensory-deprived cortices of glutamate acid decarboxylase 67 (GAD67)-green fluorescent protein (GFP) (Delta neo) and wild-type mouse. Our results showed that row D whisker trimming (WT) begun at postnatal day 7 (P7), but not after P15, induced a robust reduction of parvalbumin (PV) expression, measured by the PV/GFP ratio and PV cell densities, in the deprived barrels. WT also induced a robust reduction in the number of inhibitory perisomatic varicosities and synaptic GAD65/67 immunoreactivities in spiny neurons of the deprived barrels. Although the GAD65/67 expressions in interneurons were also downregulated in the deprived barrels, the GFP expression remained unchanged. Patch-clamp recording from spiny cells showed a 1.5-fold reduction of intracortical evoked IPSCs (eIPSCs) in deprived versus spared cortices. The reduction in eIPSCs occurred via changes in presynaptic properties and unitary IPSC amplitudes. Miniature IPSCs showed subtle but significant differences between the two experimental conditions. In addition, properties of the IPSCs in deprived barrels resemble those of IPSCs recorded in immature brains (P7). Together, these results suggest that the properties of local intracortical inhibitory networks are modified by sensory experiences. Perisomatic inhibition mediated by PV-positive basket cells is pruned by sensory deprivation.

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