期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1125, 期 1, 页码 76-88出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2006.05.019
关键词
LC-ESI-MS/MS; Escherichia coli; metabolomics; metabonomics; hydrophilic interaction chromatography; bacteria; metabolite; metabolism; triple quadrupole; mass spectrometry
A key unmet need in metabolomics is the ability to efficiently quantify a large number of known cellular metabolites. Here we present a liquid chromatography (LC)-electrospray ionization tandem mass spectrometry (ESI-MS/MS) method for reliable measurement of 141 metabolites, including components of central carbon, amino acid, and nucleotide metabolism. The selected LC approach, hydrophilic interaction chromatography with an amino column, effectively separates highly water soluble metabolites that fail to retain using standard reversed-phase chromatography. MS/MS detection is achieved by scanning through numerous selected reaction monitoring events on a triple quadrupole instrument. When applied to extracts of Escherichia coli grown in [C-12]- versus [C-13]glucose, the method reveals appropriate C-12- and C-13-peaks for 79 different metabolites. (c) 2006 Elsevier B.V. All rights reserved.
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