期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 315, 期 1-2, 页码 153-161出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2006.07.020
关键词
HLA class I; tetramer; monoclonal antibody; flow cytometry
The ability of tetrameric major histocompatibility complex (MHC) class I-peptide complexes (tetramers) to detect antigen-specific T lymphocyte responses has yielded significant information about the generation of in vivo immunity in numerous antigenic systems. Here we present a novel method for rapid validation of tetrameric HLA molecules based on the presence of allodeterminants. Human monoclonal antibodies (mAbs) recognizing polymorpbic determinants on HLA class I were immobilized on polystyrene microparticles and used to probe the structural integrity of tetrameric HLA class I molecules by flow cytometry. A total of 22 tetramers, based on HLA-A1, A2, A3, A24, B7 and B8 were reactive with their counterpart mAbs, thus confirming their antigenic integrity. A positive outcome of this mAb test ensures that tetrameric HLA class I can be used with greater confidence in subsequent functional assays. (c) 2006 Elsevier B.V. All rights reserved.
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