期刊
JOURNAL OF VIROLOGICAL METHODS
卷 136, 期 1-2, 页码 51-57出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2006.03.029
关键词
HERV-K; HIV-1; Real-Time RT-PCR; HERV-K viral load; Sybr Green
资金
- NCRR NIH HHS [G12 RR003050, G12RR03050, G12RR03050-19] Funding Source: Medline
Viral components of the human endogenous retroviruses type K (HERV-K) have been largely detected in plasma from HIV-1 infected individuals. A Sybr Green Real-Time RT-PCR approach was optimized for detection and quantitation of HERV-K RNA titers in plasma samples using the iCycler technology. The method detected 1000 HERV-K RNA copies/mL of plasma sample. The Intra- and Inter-assay performance revealed a coefficient of variations that ranged from 0.2 to 2.46%, demonstrating accuracy and reproducibility. We quantified the HERV-K RNA load in 20 HIV-1 patients receiving highly active antiretroviral therapy (HAART). We found increased HERV-K RNA titers in patients with non-suppressive HAART (patients who may develop drug-resistance and/or received suboptimal therapeutic doses), compared to suppressive regimens (p < 0.001). HERV-K RNA was not detected in HCV-1 positive or seronegative controls. Sequencing of Real-Time RT-PCR products revealed particular HERV-K subtypes activated in the HIV-1 infection. The application of this assay could expand the understanding of the role of HERV-K in the HIV-1 infection and others pathological conditions. (c) 2006 Elsevier B.V. All rights reserved.
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