期刊
NATURE METHODS
卷 3, 期 9, 页码 745-751出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth910
关键词
-
资金
- NHLBI NIH HHS [N01-HV-28183] Funding Source: Medline
- NIAID NIH HHS [AI50854, AI50865] Funding Source: Medline
- NIAMS NIH HHS [AR49328] Funding Source: Medline
- NIDDK NIH HHS [DK61934] Funding Source: Medline
Antigen microarrays hold great promise for profiling the humoral immune response in the settings of autoimmunity, allergy and cancer. This approach involves immobilizing antigens on a slide surface and then exposing the array to biological fluids containing immunoglobulins. Although these arrays have proven extremely useful as research tools, they suffer from several sources of variability. To address these issues, we have developed a new two-color Fab labeling method that allows two samples to be applied simultaneously to the same array. This straightforward labeling approach improves reproducibility and reliably detects changes in autoantibody concentrations. Using this technique we profiled serum from a mouse model of systemic lupus erythematosus (SLE) and detected both expected and previously unrecognized reactivities. The improved labeling and detection method described here overcomes several problems that have hindered antigen microarrays and should facilitate translation to the clinical setting.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据