IL-10 is essential for disease protection following intranasal peptide administration in the C57BL/6 model of EAE

We have shown previously that intranasal administration of encephalitogenic peptides in soluble form to H-2(u) and H-2(s) mice affords protection from experimental autoimmune encephalomyelitis (EAE). Here we demonstrate that this method of disease protection can be induced in C57BL/6 mice by administration of the soluble peptide 35-55 from myelin oligodendrocyte glycoprotein. This protective effect was demonstrated by the evaluation of both clinical EAE scores and central nervous system histopathology; the latter showing minimal inflammatory infiltrates in treated mice. The employment of an IL-10(-/-) congenic strain allowed an appraisal of the involvement of IL-10 in this process. The lack of disease protection in these mice clearly demonstrates the non-redundant role of IL-10 in this process. (c) 2006 Elsevier B.V. All rights reserved.