期刊
JOURNAL OF MAGNETIC RESONANCE
卷 182, 期 1, 页码 173-177出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2006.06.009
关键词
DPFGSE NOE; DPFGSE ROE; initial rate approximation; NOE buildup; peak amplitude normalization for improved cross-relaxation; PANIC
The nuclear Overhauser effect (NOE) is undoubtedly one of the most useful tools in NMR spectroscopy and is widely used in solving structural and conformational problems of small organic molecules and macromolecular systems alike. In particular, measurement of the kinetics of the NOE, often facilitated by selective ID NOE buildup experiments, can generate invaluable quantitative distance information for the molecule being investigated. In practice, analysis of such kinetic NOE data routinely assumes a first-order approximation of the initial buildup rate. However, often times such an approximation holds true only for the shortest mixing times. As shown by Macura and others, the linear range of the NOE buildup obtained from 2D NOESY and exchange experiments can be substantially extended by simply scaling the NOE cross-peaks against the corresponding diagonal peaks. In this note, we demonstrate through a detailed analysis that the same approach can be applied to the analysis of ID NOE data obtained with the DPFGSE NOE pulse sequence, one of the most widely used selective ID NOE experiments today. We show that this approach allows the inclusion of data points acquired with much longer mixing times in the analysis and thus considerably improves the accuracy of the measured cross-relaxation rates and internuclear distances, while considerably simplifying the data analysis. Similar results can be obtained for the rotating frame DPFGSE ROE experiment. (c) 2006 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据