期刊
PLANT CELL AND ENVIRONMENT
卷 29, 期 9, 页码 1686-1702出版社
WILEY
DOI: 10.1111/j.1365-3040.2006.01542.x
关键词
microarray analysis; ozone; real-time RT-PCR; RLKs; transcription factors; WRKY proteins
To analyse cellular response to O-3, the tolerant Arabidopsis thaliana genotype Col-0 was exposed to O-3 fumigation (300 ppb) for 6 h and the modulation of gene expression during the treatment (3 h after the beginning of the treatment, T3 h) and the recovery phase (6 h from the end of the treatment, T12 h) assessed by gene chip microarray and real-time reverse transcriptase (RT)-PCR analyses. The Arabidopsis transcriptional profile is complex, as new genes (i.e. reticuline oxidase) and pathways, other than those already reported as O-3-responsive, appear to be involved in the O-3 response. The steady-state transcript levels of several WRKY genes were increased in O-3-treated plants and the W-box was the cis-element over-represented in the promoter region of T3 h up-regulated genes. The fact that the W-box element was also over-represented in almost all T3 h-induced receptor-like kinases (RLKs) suggests a WRKY-mediated control of RLKs under O-3 stress and a mechanicistic similarity with the pathogen-induced transcriptional responses. We investigated the molecular and physiological implications of our findings in relation to O-3-induced plant stress response.
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