期刊
CYTOMETRY PART A
卷 69A, 期 9, 页码 1037-1042出版社
WILEY-LISS
DOI: 10.1002/cyto.a.20333
关键词
control; gating; specificity; compensation; instrumentation setup
A frequent goal of flow cytometric analysis is to classify cells as positive or negative for a given marker, or to determine the precise ratio of positive to negative cells. This requires good and reproducible instrument setup, and careful use of controls for analyzing and interpreting the data. The type of controls to include invarious kinds of flow cytometry experiments is a matter of some debate and discussion. In this tutorial, we classify controls in various categories, describe the options within each category, and discuss the merits of each option.
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