期刊
GLIA
卷 54, 期 4, 页码 316-328出版社
WILEY-BLACKWELL
DOI: 10.1002/glia.20384
关键词
astrocyte; glia; resting membrane potential; inward rectifying potassium channels; Kir 4.1; cAMP; protein kinase A
The factors regulating astroglial resting membrane potential (RMP) are unresolved. Here, we have examined developmental, morphological, and intracellular factors that may regulate the RMP in astrocytes of isolated intact optic nerves of rats and mice aged postnatal day (P3) to adult. The astroglial RMP ranged from -25 to -85 mV, independent of age and morphological phenotype. There was a developmental negative shift in the astroglial RMP from a non-Gaussian distribution in perinatal nerves, to a bimodal distribution of RMPs after P15, with peaks at -52 and -74 mV in adults. Blockade of Kir with 100 mu M BaCl2 significantly depolarized the RMP to -30 mV; the RMP was unaffected by TEA or agents that modulated ATP-sensitive potassium channels. Raising intracellular cyclic AMP (CAMP) with dbcAMP or forskolin induced a significant hyperpolarization by similar to 15 mV whereas inhibition of cAMP-dependent protein kinase (PKA) depolarized the astroglial RMP to -40 mV The hyperpolarizing action of dbcAMP was blocked by 100 mu M BaCl2. The effects of BaCl2 indicate that the developmental negative shift in the RMP and the cAMP-mediated hyperpolarization were dependent on Kir. This study provides evidence that the heterogeneous RMP of mature astrocytes is regulated by cAMP and PKA signaling. (c) 2006 Wiley-Liss, Inc.
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