期刊
GENESIS
卷 44, 期 9, 页码 407-411出版社
WILEY-LISS
DOI: 10.1002/dvg.20221
关键词
jumonji (Jarid 2); Cre-IoxP technology; conditional knockout; mouse development; heart
资金
- NHLBI NIH HHS [R01 HL067050, R01 HL067050-01A1, R01 HL067050-05A1, HL67050, R01 HL067050-02, R01 HL067050-03, R01 HL067050-04] Funding Source: Medline
The jumonji (jmj) gene plays important roles in multiple organ development in mouse, including cardiovascular development. Since JMJ is expressed widely during mouse development, it is essential that conditional knockout approaches be employed to ablate JMJ in a tissue-specific manner to identify the cell lineage specific roles of JMJ. In this report, we describe the establishment of a jmj conditional null allele in mice by generating a loxP-flanked (floxed) jmj allele, which allows the in vivo ablation of jmj via Cre recombinase-mediated deletion. Gene targeting was used to introduce IoxP sites flanking exon 3 of the jmj allele to mouse embryonic stem cells. Our results indicate that the jmj floxed allele converts to a null allele in a heart-specific manner when embryos homozygous for the floxed jmj allele and carrying the alpha-myosin heavy chain promoter-Cre transgene were analyzed by Southern and Northern blot analyses. Therefore, this mouse line harboring the conditional jmj null allele will provide a valuable tool for deciphering the tissue and cell lineage specific roles of JMJ.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据