期刊
NEUROBIOLOGY OF AGING
卷 27, 期 9, 页码 1224-1238出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.neurobiolaging.2005.06.007
关键词
Alzheimer's disease; amyloid peptide; cholinergic neurons; axonal degeneration; neurodegenerative diseases
The goal of this study was to assess if neurons exposed to amyloid-beta peptide (A beta) exclusively in distal axons, undergo apoptosis. This is relevant to the loss of cholinergic neurons in Alzheimer's disease. Using a three-compartmented culture system for rat sympathetic neurons, we demonstrate that exposure of axons to A beta(1-42) activates an independent destruction program in axons, which leads to nuclear apoptosis. A beta-induced axonal degeneration does not involve local caspase activation, but causes caspase activation in cell bodies. Accordingly, inhibition of caspase activation blocks A beta-induced apoptosis but not axonal degeneration. In agreement with previous suggestions that disruption of nerve growth factor (NGF)-mediated signaling might contribute to the loss of cholinergic neurons, we found that provision of NGF to cell bodies protects sympathetic neurons from A beta-induced apoptosis. However, our data indicate that A beta-induced axonal degeneration follows a mechanism different than that activated by NGF withdrawal. Only A beta-induced axonal degeneration is prevented by the calpain inhibitor calpastatin and is insensitive to the inhibitor of the ubiquitin-proteasome system MG132. Importantly, inhibition of A beta-induced axonal degeneration by calpastatin prevents nuclear apoptosis. (c) 2005 Published by Elsevier Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据