期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 72, 期 9, 页码 6371-6376出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00701-06
关键词
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Phylogenetic analysis was used to compare 16S rRNA sequences from 19 cultured human gut strains of Roseburia and Eabacterium rectale with 356 related sequences derived from clone libraries. The cultured strains were found to represent five of the six phylotypes identified. A new oligonucleotide probe, Rrec584, and the previous group probe Rint623, when used in conjunction with a new helper oligonucleotide, each recognized an average of 7% of bacteria detected by the eubacterial probe Eub338 in feces from 10 healthy volunteers. Most of the diversity within this important group of butyrate-producing gut bacteria can apparently be retrieved through cultivation.
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