期刊
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
卷 34, 期 5, 页码 515-521出版社
INFORMA HEALTHCARE
DOI: 10.1080/10731190600862886
关键词
ATP-regeneration; immobilization; inclusion bodies; polyphosphate kinase
Active inclusion bodies of recombinant polyphosphate kinase were obtained by simple washing of Escherichia coli cells with nonionic detergent and then they were immobilized in agar/TiO2 beads. Bioenergy beads obtained are charged by polyphosphate to act as rechargeable suppply of adenosine/nucleoside triphosphates (ATP/NTP), a practical tool for synthesis of artificial receptors.
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