期刊
NEUROPHARMACOLOGY
卷 51, 期 4, 页码 816-821出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuropharm.2006.05.027
关键词
chemokines; mouse hippocampus; long-term depression; fEPSP
This work reports the effect of chemokine fractalkine/CX(3)CL1, an endogenous small peptide highly expressed in the central nervous system, on evoked synaptic responses investigated in mouse CA1 stratum radiatum using an electrophysiological approach. We report that in acute mouse hippocampal slices, superfusion of CX(3)CL1 resulted in a reversible depression of the field excitatory postsynaptic potential (fEPSP) which developed within few seconds, increased for up to 10 min of application and disappeared within 30 min after the end of CX(3)CL1 treatment. We also show that CX(3)CL1-induced synaptic depression is (i) dose-dependent with IC50 and n(H) values of 0.7 nM and 1, respectively, (ii) not associated with a change in paired-pulse facilitation, (iii) mediated through CX(3)CL1 receptor (CX(3)CR1), being absent in CX(3)CR1(-/-) mice and inhibited in wild-type mice by a specific blocking antibody, and (iv) occluded by the induction of homosynaptic long-term depression (LTD). We conclude that CX(3)CL1 is a potent neuromodulator of the evoked excitatory synaptic transmission, sharing common mechanisms with LTD. (c) 2006 Elsevier Ltd. All rights reserved.
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