Structure of a teichoic acid-like O-polysaccharide of Escherichia coli O29

A teichoic acid-like O-polysaccharide was isolated by mild acid degradation. of the lipopolysaccharide (LPS) of Escherichia coli O29. The O-polysaccharide and an oligosaccharide obtained by dephosphorylation of the O-polysaccharide were studied by sugar analysis along with H-1 and C-13 NMR spectroscopy. The following structure of the branched oligosaccharide repeating unit, containing five monosaccharide residues and glycerol 1-phosphate (D-Gro-1-P), was established: alpha-D-Glcp-(1 -> 6)-alpha-D-Galp 1 down arrow 3 -> 6)-beta-D-Glcp-(1 -> 4)-alpha-L-FucpNAc-(1 -> 3)-beta-D-GLcpNAc-(1 -> 3)-D-Gro-1-P-(O -> (c) 2006 Elsevier Ltd. All rights reserved.