期刊
BIOSENSORS & BIOELECTRONICS
卷 22, 期 3, 页码 409-414出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2006.05.002
关键词
dioxin-BGG; anti-dioxin monoclonal antibody; ScFv; QCM
To detect dioxin using a quartz crystal microbalance (QCM) immunosensor, anti-2,3,7,8-tetrachloro-p-dibenzodioxin (TCDD) monoclonal antibodies (MAbs) were produced as types of IgGI and IgM, with mono 6-(2,3,6,7-tetrachloroxanthene-9-ylidene) hexyl succinate (as a hapten) conjugated with bovine serum albumin (dioxin-BSA). Furthermore, ScFv was generated from hybridoma-producing IgGI MAb. Among these antibodies, ScFv showed excellent capability for dioxin detection using QCM immunosensors. (c) 2006 Elsevier B.V. All rights reserved.
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