Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles

A novel, automated system, PNE- 1080, equipped with eight automated pestle units and a spectrophotometer was developed for genomic DNA extraction from maize using ammosilane-modified bacterial magnetic particles (BMPs). The use of aminosilane-modified BMPs allowed highly accurate DNA recovery. The (A(260)-A(320)): (A(280)-A(320)) ratio of the extracted DNA was 1.9 +/- 0.1. The DNA quality was sufficiently pure for PCR analysis. The PNE- 1080 offered rapid assay completion (30 min) with high accuracy. Furthermore, the results of real-time PCR confirmed that our proposed method permitted the accurate determination of genetically modified DNA composition and correlated well with results obtained by conventional cetyltrimethylammonium bromide (CTAB)-based methods. (c) 2006 Elsevier B.V. All rights reserved.