期刊
JOURNAL OF CONTROLLED RELEASE
卷 115, 期 1, 页码 18-25出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2006.06.031
关键词
self-assembling peptides; nanofiber scaffold hydrogel; diffusion coefficient; controlled molecule release
Biological hydrogels consisting of self-assembling peptide nanofibers are potentially excellent materials for various controlled molecular release applications. The individual nanofiber consists of ionic self-complementary peptides with 16 amino acids (RADA16, Ac-RADARADARADAPADA-CONH2) that are characterized by a stable P-sheet structure and undergo self-assembly into hydrogels containing similar to 99.5% w/v water. We report here on the diffusion properties of phenol red, bromophenol blue, 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (pyranine, 3-PSA), 1,3,6,8-pyrenctetrasulfonic acid tetrasodium salt (4-PSA), and Coomassie Brilliant Blue G-250 (CBBG) through RADA16 hydrogels. The apparent diffusivity (D) of phenol red (1.05 +/- 0.08 x 10(-10) m(2) s(-1)) is higher than that of 3-PSA (0.050 +/- 0.004 x 10(-10) m(2) s(-1)) and 4-PSA (0.007 +/- 0.002 x 10(-10) m(2) s(-1)). The difference in 3-PSA and 4-PSA diffusivities suggests that the sulfonic acid groups directly facilitate electrostatic interactions with the RADA16 fiber surface. Bromophenol blue and CBBG were not released from the hydrogel, suggesting that they interact strongly with the peptide hydrogel scaffold. The ditfusivities (D) of the dyes decreased with increasing hydrogel peptide concentration, providing an alternate route of controlling release kinetics. These results indicate that release profiles can be tailored through controlling nanofiber-diffusant molecular level interactions. (c) 2006 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据