期刊
ANNALS OF DERMATOLOGY
卷 23, 期 2, 页码 138-143出版社
KOREAN DERMATOLOGICAL ASSOC
DOI: 10.5021/ad.2011.23.2.138
关键词
beta-catenin; Outer root sheath cells; Sox9
类别
资金
- National Research Foundation of Korea [2009-0075011]
- National Research Foundation of Korea [2009-0075011] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Background: beta-catenin plays a pivotal role in hair follicle development and hair growth cycle. Objective: The aim of this study was to identify beta-catenin-regulated genes in cultured human hair outer root sheath (ORS) cells. Methods: Primary cultured ORS cells were transduced with recombinant adenovirus expressing N-terminal truncated beta-catenin (constitutive active form), and beta-catenin-regulated genes were identified. Results: Overexpression of the constitutively active form of beta-catenin led to induction of Sox9 expression at both mRNA and protein levels. To investigate the potential role of Sox9, we made the recombinant adenovirus expressing green fluorescent protein-tagged Sox9, and then transduced into cultured ORS cells. Interestingly, Sox9 induced the expression of keratin 15, increased the proliferation of ORS cells in vitro, and enhanced colony-forming activity. Conclusion: Our results suggest that Sox9 is a R-catenin-regulated gene in ORS cells, and has potential importance in the regulation of hair follicle homeostasis. (Ann Dermatol 23(2) 138 similar to 143, 2011)
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