期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 386, 期 3, 页码 639-644出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-006-0559-2
关键词
gold nanoparticle; localized surface plasmon resonance; biomolecular sensing; antibody; serum albumin; protein immobilization
Antibody binding to bovine serum albumin (BSA) and human serum albumin (HSA) immobilized onto gold nanoparticles was studied by means of localized surface plasmon resonance (LSPR) spectroscopy. Amine-modified glass was prepared by self-assembly of amine-terminated silane on substrate, and gold (Au) nanoparticles were deposited on the amine-modified glass substrate. Au nanoparticles deposited on the glass surface were functionalized by BSA and HSA. BSA immobilization was confirmed by LSPR spectroscopy in conjunction with surface-enhanced Raman scattering spectroscopy. Then, LSPR response attributable to the binding of anti-BSA and anti-HSA to BSA- and HSA-functionalized Au nanoparticles, respectively, was examined. Anti-HSA at levels larger than similar to 10 nM could be detected by HSA-immobilized chips with LSPR optical response, which was saturated at concentrations greater than similar to 650 nM of anti-HSA.
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