期刊
BIOMATERIALS
卷 27, 期 7, 页码 1095-1103出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2005.08.015
关键词
cartilage defects; gene-activated matrix; transforming growth factor-beta 1; chitosan; gelatin
Cartilage defects as a result of disease or injury have a very limited ability to heal spontaneously. Recently, tissue engineering and local therapeutic gene delivery systems have been paid much attention in the cartilage natural healing process. Gene-activated matrix (GAM) blends these two strategies, serving as local bioreactor with therapeutic agents expression and also providing a structural template to fill the lesion defects for cell adhesion, proliferation and synthesis of extracellular matrix (ECM). In the current study, we used chitosan-gelatin complex as biomaterials to fabricate three-dimensional scaffolds and plasmid DNA were entrapped ill the scaffolds encoding transforming growth factor-beta 1 (TGF-beta 1), which has been proposed as a promoter of cartilage regeneration for its effect on the synthesis of matrix molecules and cell proliferation. The plasmid DNA incorporated in the scaffolds showed a burst release in the first week and a sustained release for the other 2 weeks. The gene transfectd into chondrocytes expresses TGF-beta 1 protein stably in 3 weeks. The histological and immunohistochemical results confirmed that the primary chondrocytes cultured into the chitosan-gelatin scaffold maintained round and owned characters of high secretion of specific ECM. From this Study, it call be concluded that this gene-activated chitosan-gelatins matrix has a potential in the application of cartilage defects regeneration. (c) 2005 Elsevier Ltd. All rights reserved.
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