期刊
EXPERIMENTAL HEMATOLOGY
卷 34, 期 10, 页码 1333-1343出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.exphem.2006.05.023
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资金
- NHLBI NIH HHS [1P50 HL54850] Funding Source: Medline
- NIAID NIH HHS [N01 AI30070] Funding Source: Medline
Objective. Methods of gene transfer to hematopoietic stem cells that result in stable integration may provide treatments for many inherited and acquired blood diseases. It has been demonstrated previously that a gene delivery system based on the Sleeping Beauty (SB) transposon can be derived where a plasmid transiently expressing the SB transposase can mediate the stable chromosomal integration of a codelivered second plasmid containing a gene expression unit flanked by the inverted repeats derived from the transposon. Methods. Plasmid DNA containing the elements required for SB transposition was delivered to hematopoietic cells via electroporation. Integrated transgene (enhanced green fluorescent protein [eGFP]) expression was assessed in vitro and in vivo. Results. In the K562 human hematopoietic cell line, we observed stable expression of eGFP in > 60% of cells for over 2 months after electroporation of the two plasmids; in contrast, in control cells either not treated with transposase or exposed to a defective mutant transposase, the level of gene expression had fallen to near background (< 0.1%) by 2 weeks. In purified human cord blood CD34(+) progenitor cells, the transposase led to stable gene transfer at levels up to 6% for over 4 weeks, but gene transfer to more primitive nonobese diabetic/severe combined immunodeficient repopulating cells or CD34(+)/CD38(-) in long-term culture was low and electroporation of the cells with plasmid DNA caused significant cell death. Conclusion. The long-term stable expression highlights the potential of this transposase-based gene delivery method for ameliorating diseases affecting the hematopoietic system, although further improvements in gene transfer efficacy are needed. (c) 2006 International Society for Experimental Hematology. Published by Elsevier Inc.
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