期刊
ANNALS OF BOTANY
卷 107, 期 7, 页码 1183-1192出版社
OXFORD UNIV PRESS
DOI: 10.1093/aob/mcq142
关键词
Arabidopsis thaliana; cell-cycle checkpoints; hydroxyurea; root growth; NaCl; zeocin
资金
- Cardiff University
- Worcester University
- Calabria University
- John Innes Centre
- Biotechnology and Biological Sciences Research Council [BBS/E/J/00000129] Funding Source: researchfish
Background and Aims In yeasts and animals, cyclin-dependent kinases are key regulators of cell cycle progression and are negatively and positively regulated by WEE1 kinase and CDC25 phosphatase, respectively. In higher plants a full-length orthologue of CDC25 has not been isolated but a shorter gene with homology only to the C-terminal catalytic domain is present. The Arabidopis thaliana; CDC25 can act as a phosphatase in vitro. Since in arabidopsis, WEE1 plays an important role in the DNA damage/DNA replication checkpoints, the role of Arath;CDC25 in conditions that induce these checkpoints or induce abiotic stress was tested. Methods arath;cdc25 T-DNA insertion lines, Arath; CDC25 over-expressing lines and wild type were challenged with hydroxyurea (HU) and zeocin, substances that stall DNA replication and damage DNA, respectively, together with an abiotic stressor, NaCl. A molecular and phenotypic assessment was made of all genotypes Key Results There was a null phenotypic response to perturbation of Arath; CDC25 expression under control conditions. However, compared with wild type, the arath; cdc25 T-DNA insertion lines were hypersensitive to HU, whereas the Arath; CDC25 over-expressing lines were relatively insensitive. In particular, the over-expressing lines consistently outgrew the T-DNA insertion lines and wild type when challenged with HU. All genotypes were equally sensitive to zeocin and NaCl. Conclusions Arath; CDC25 plays a role in overcoming stress imposed by HU, an agent know to induce the DNA replication checkpoint in arabidopsis. However, it could not enhance tolerance to either a zeocin treatment, known to induce DNA damage, or salinity stress.
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