期刊
ANNALS OF BOTANY
卷 103, 期 5, 页码 807-817出版社
OXFORD UNIV PRESS
DOI: 10.1093/aob/mcn267
关键词
Arabidopsis thaliana; auxin; lateral root density; lateral root initiation index; mutant phenotype; pericycle; root architecture; root branching; root primordium; Solanum lycopersicum
资金
- US Department of Agriculture National Research Initiative Competitive [2006-03434]
- Direccion General de Asuntos del Personal Academico de la Universidad Nacional Automa de Mexico
- Czech Ministry of Youth, Education and Sports [LC06034]
- Programa de Apoyo a Proyectos de Investigacion e Innovacion Tecnologica de la Universidad Nacional Automa de Mexico [IN225906]
- Consejo Nacional de Ciencia y Tecnologia of Mexico (CONACyT) [49267]
- CONACyT-Secretaria de Medio Ambiente y Recursos Naturales of Mexico [2004-C01-80]
Lateral root initiation is an essential and continuous process in the formation of root systems; therefore, its quantitative analysis is indispensable. In this study a new measure of lateral root initiation is proposed and analysed, namely the lateral root initiation index (I-LRI), which defines how many lateral roots and/or primordia are formed along a parent-root portion corresponding to 100 cortical cells in a file. For data collection, a commonly used root clearing procedure was employed, and a new simple root clearing procedure is also proposed. The I-LRI was determined as 100dl, where d is the density of lateral root initiation events (number mm(-1)) and l is the average fully elongated cortical cell length (mm). Analyses of different Arabidopsis thaliana genotypes and of a crop plant, tomato (Solanum lycopersicum), showed that I-LRI is a more precise parameter than others commonly used as it normalizes root growth for variations in cell length. Lateral root primordium density varied in the A. thaliana accessions Col, Ler, Ws, and C24; however, in all accessions except Ws, I-LRI was similar under the same growth conditions. The nitrogen/carbon ratio in the growth medium did not change the lateral root primordium density but did affect I-LRI. The I-LRI was also modified in a number of auxin-related mutants, revealing new root branching phenotypes in some of these mutants. The rate of lateral root initiation increased with Arabidopsis seedling age; however, I-LRI was not changed in plants between 8 and 14 d post-germination. The I-LRI allows for a more precise comparison of lateral root initiation under different growth conditions, treatments, genotypes and plant species than other comparable methods.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据