期刊
BIOLOGY OF REPRODUCTION
卷 75, 期 4, 页码 624-632出版社
OXFORD UNIV PRESS INC
DOI: 10.1095/biolreprod.106.050732
关键词
circadian rhythm; gene regulation; luteinizing hormone; mechanisms of hormone action; ovary
资金
- NIEHS NIH HHS [ES012948] Funding Source: Medline
A molecular device that measures time on a daily, or circadian, scale is a nearly ubiquitous feature of eukaryotic organisms. A core group of clock genes, whose coordinated function is required for this timekeeping, is expressed both in the central clock and within numerous peripheral organs. We examined expression of clock genes in the rat ovary. Transcripts for core oscillator elements (Arntl, Clock, Per1, Per2, and Cry1) were present in the ovary as indicated by quantitative real-time RT-PCR. Rhythmic expression patterns of Arnt1 and Per2 transcripts and protein products were out of phase with respect to the central oscillator and in complete antiphase to each other. Expression of Arnt1 was significantly elevated after the LH surge on the day of proestrus. Finally, hCG treatment induced cyclic expression of both Arnt1 and Per2 gene products in hypophy-sectomized, immature rats primed with eCG. Collectively, these data suggest that the core underpinnings of the transcriptional/translational feedback loop that drives circadian rhythmicity is present in the rat ovary. Furthermore, the study identifies LH as a potential regulator of circadian clock gene rhythms in the ovary.
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