期刊
PLANT BREEDING
卷 125, 期 5, 页码 488-492出版社
WILEY
DOI: 10.1111/j.1439-0523.2006.01284.x
关键词
Cucumis sativus; F-1 hybrid; genetic marker; genetic distance; genotyping; polymorphism; RT-PCR; sequence variation
A cDNA-AFLP transcript profiling was employed to examine three representative tissues (seedling, ovary and leaf) of nine Korean cucumber (Cucumis sativus L.) F-1 hybrids. Differential accumulation of transcript-derived fragments (TDFs) was detected in 92 profiles. Genetic distance-based cluster analysis partitioned these hybrids into four main groupings, consistent with their phenotypic relationships. Although several polymorphic profiles were confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) analysis, many were not reproducible, indicating that a large portion of the observed polymorphisms were based on sequence variation of transcripts rather than expression of variation. Thus, it is proposed that cDNA-AFLP profiling be based on a dual descriptor system (sequence and expression). Data indicate that such a system would provide an efficient genetic marker system for identifying polymorphisms in narrow genetic pools.
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