期刊
ANNALS OF BIOMEDICAL ENGINEERING
卷 41, 期 6, 页码 1208-1216出版社
SPRINGER
DOI: 10.1007/s10439-013-0791-9
关键词
Robotic cell manipulation; Visual servoing; Biological cell characterization; Micropipette aspiration; Mechanical properties
资金
- Natural Sciences and Engineering Research Council of Canada
- Canada Research Chairs Program
This paper presents a system for mechanically characterizing single cells using automated micropipette aspiration. Using vision-based control and position control, the system controls a micromanipulator, a motorized translation stage, and a custom-built pressure system to position a micropipette (4 mu m opening) to approach a cell, form a seal, and aspirate the cell into the micropipette for quantifying the cell's elastic and viscoelastic parameters as well as viscosity. Image processing algorithms were developed to provide controllers with real-time visual feedback and to accurately measure cell deformation behavior on line. Experiments on both solid-like and liquid-like cells demonstrated that the system is capable of efficiently performing single-cell micropipette aspiration and has low operator skill requirements.
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